Extended Data Fig. 6: Synthetic ssDNA cleavage by TnpB RNP complex.
From: Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease
a, b, Cleavage of ssDNA substrates containing a target (represented in green colour) with TAM (red colour) (a) or without TAM sequence (b). TnpB RNP complex cleaves ssDNA in a TAM-independent manner. Cleavage is blocked by D191A mutation at the RuvC-like active site (lane D). NTS and TS represent non-target and target strand, respectively. M – DNA marker lane. For uncropped gel images, see Supplementary Fig. 2.
