Fig. 4: TnpB nuclease is a novel genome editor.
From: Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease
a, The experimental workflow of the human cell line (HEK293T) genome-editing experiment. NLS, nuclear localization sequence. b, Detection of indel activity in the five tested targets of 20 nt in length in human gDNA (represented as the mean of three biologically independent experiments (shown in dots) ± standard deviation). The TnpB (non-targeting) expression plasmid used as a negative control encodes the reRNA-containing guide sequence that does not match any target in the human gDNA. c, Indel profile distribution within the target sequence in the EMX1-1 site showing the distribution of deletions (blue line) and insertions (red line) across the cleavage site (dotted line). The profile was obtained by aligning all reads at the EMX1-1 site and counting deletions and insertions at each position.
