Extended Data Fig. 3: Position frequency matrix (PFM) of the nucleotides at 7N region obtained after plasmid library cleavage using E. coli lysates.
From: Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease
a, b, PFM representation of nucleotides distribution identified in 7N randomized region at 20–21 bp F + R1 enriched adapter ligated reads obtained from E. coli cells expressing TnpB and reRNA constructs with 16 nt and 20 nt guide sequences, respectively. c, the cleavage reaction using lysate obtained from the cells that do not express TnpB demonstrates no substantial enrichment of motifs at 7N region.
