Extended Data Fig. 15: a. Tetramerized 10LH binds to PHOX2B pMHC.
From: RETRACTED ARTICLE: Cross-HLA targeting of intracellular oncoproteins with peptide-centric CARs
Pulsing SW620 HLA-A*24:02 with 50μM PHOX2B peptide results in 10LH tetrabody binding. b. CARs predicted to be cross-reactive show killing in HLA-matched cells. CARs 320LH and 280LH predicted to cross-react with peptides presented on normal tissue demonstrate significant cross-reactivity in HLA-matched SW620 cells. Representative images shown from n = 3 technical replicates.
