Extended Data Fig. 7: Reproducibility of SWR suppression of cue-responsive CA3SCs with automated and manual sparse ROI segmentation and cell body ROI.
From: Adaptive stimulus selection for consolidation in the hippocampus
a, Left, representative time-averaged FOV from one representative mouse (n=6 in this study). Right, zoomed-in patches of FOV on left (yellow dashed square) showing original (default Suite2p performance), sparse automated, and sparse manual segmentation (Methods). ROIs overlaid onto FOV in random transparent colors at top with ROI outlines plotted below. b, Cumulative histogram of Euclidean distance between each ROI and its nearest neighboring ROI (black: n=7055 original ROIs; pink: n=2945 sparse automated ROIs). Median values±s.d. are shown in bar plot (inset). c, Density of surrounding ROIs calculated for each ROI as a function of distance. d–g, Analysis of automatically-detected CA3SC ROIs including mean cue responses±s.e.m. of cue-CA3SCs during RC-NS (d), mean peri-SWR response±s.e.m. of all cue-CA3SCs (Cue, red) and non-cue CA3SCs (Other, black) recorded (e), fraction of cue-responsive and non-cue CA3SCs (910 cue-CA3SC ROIs from 6 mice) (f), and fraction of SWR recruitment of CA3SCs during RC-NS and POST (Other: n=1,945; Cue: n=910 ROIs from 6 mice. two-sided Fisher’s exact test with Bonferroni correction. P=2.6x10−24 for Cue-vs-All and Cue-vs-Other) (g). h–k, Analysis of manually-curated sparse CA3SC ROIs plotted as in (d-g) (Other: n=1,083; Cue: n=255 ROIs from 6 mice. Fisher’s exact test with Bonferroni correction. P=1.2x10−7 for Cue-vs-All and Cue-vs-Other). Fraction of hand-drawn cue-responsive and non-cue CA3SC ROIs. k, Fraction of SWR recruitment. l, Schematic of window implantation above CA3 stratum pyramidale and CA3 pyramidal cells injected with rAAV2/1:Syn(GCaMP7f)Cre in Grik4-Cre mice. A 4-channel linear silicon probe was implanted in contralateral CA1. m, Example motion-corrected, time-averaged two photon imaging FOV from one representative mouse (n=6 in this study). Scale bar=100 μm n, Left, two example Suite2p-detected ROIs from (b). Right, representative GCaMP calcium signals (ΔF/F0) extracted from CA3 pyramidal cell ROIs. o, Mean cue responses±s.e.m. of identified cue-CA3 cells during RC-NS in n=6 mice. p, Mean peri-SWR response±s.e.m. of cue- and non-cue-CA3 cells recorded during RC-NS. q, Fraction of cue-responsive and non-cue CA3 cells in RC-NS. r, Fraction of SWR recruitment during RC-NS and POST (Other: n=238; Cue: n=76 ROIs from 6 mice. two-sided Fisher’s exact test with Bonferroni correction. P=2.7x10−9 for Cue-vs-All and Cue-vs-Other).
