Extended Data Fig. 2: Stability of two-photon imaging.
From: Adaptive stimulus selection for consolidation in the hippocampus
a, Top, cue onsets in a representative RC-NS session (orange: odor; green: visual; light blue: reward). Bottom, trial-averaged FOVs corresponding to each odor trial from one representative mouse (n=6 in this study). Trial averages were computed from the frame of odor stimulus onset through the last frame 5 s prior to the next stimulus onset. b, Mean pixel-wise correlations between all pairs of trial-averaged FOVs from the above session. c, Mean pixel-wise correlations of trial-averaged FOVs with whole-session time-averaged FOVs in RC-NS sessions. Colors correspond to individual mice. Correlations were recalculated with whole-session time-averaged FOVs on different mice (shuffled) and plotted in black (mean, shaded area: 90% confidence interval). d, Left, empirical cumulative distribution function (CDF) of mean squared error (MSE) between each frame and time-averaged FOVs from corresponding sessions (green: all frames, orange: intra-trial frames, black: comparison to other FOVs). Middle, violin plot of data shown in CDFs. Right, MSEs between each frame and trial-averaged FOVs. MSEs were averaged every 10 trials and shaded areas indicate percentiles from 5% to 95%. e–i, Same as (a) for each individual mouse.
