Extended Data Fig. 4: Flow chart for the cryo-EM data processing and structure determination of the dolphin prestin in SO42−.
From: The conformational cycle of prestin underlies outer-hair cell electromotility
a, b Cryo-EM data processing and structure determination of the dolphin prestin in Down I (SO42−) and Down II (SO42−) states. A was obtained from Dataset I, which was combined with Class B from Dataset II. The final reconstruction yielded two structures, Down I (SO42−) and Down II (SO42−), which have nominal resolutions of 4.2 and 6.7 Å, respectively (at FSC=0.143). See Supplementary Figure 5 for the steps on how Class A and B were further processed. Evidence of both states was found in dataset II, however merging of datasets was required to improve resolution of states. c, Flow chart for the cryo-EM data processing and structure determination of the dolphin prestin in the Intermediate state (SO42−) (See Methods for details). The final reconstruction has a nominal resolution of 4.6 Å (at FSC=0.143). UCSF ChimeraX was for illustration of all the structures. The yellow scale bar on all the micrographs represents 200 Å.
