Extended Data Fig. 2: Characterisation and comparison of a CS7 human gastrula with Non-human primate and Mouse.

a, Heatmap with the normalized log expression of well characterized marker genes for the identified cell types: Epiblast (Epi), Ectoderm (Amniotic/Embryonic (EAE)), Primitive Streak (PS), Nascent Mesoderm (NM), Emergent Mesoderm (EM), Advanced Mesoderm (AM), Extraembryonic Mesoderm (ExM), Axial Mesoderm (AxM), Endoderm (Endo), Hemato-Endothelial Progenitors (HEP), Erythroblasts (Ery). b, Stacked bar plots highlighting the anatomical region that cells were collected from and the percentage breakdown of each cluster. Numbers in brackets represent the total number of cells per cluster. c, Heatmap showing the fraction of human gastrula cells allocated to mouse cell types at E7.25 (data from¹⁸). d, Dendrogram showing hierarchical clustering of the transcriptomes of cell types from human gastrula and cultured cynomolgus macaque embryos at 16-day post-fertilization (from¹⁹). e, Top, UMAP plots showing the log expression of MEST and GCNT2. Bottom, violin plots showing the log expression of total transcripts (top row) and selected isoforms scaled by the maximum value in different cell types. Isoform names refer to Ensembl nomenclature.