Extended Data Fig. 6: Immunodepletion of clusterin in runner plasma abrogates its anti-inflammatory properties on the hippocampus.
From: Exercise plasma boosts memory and dampens brain inflammation via clusterin
a. Male mice (3-4 months of age) were injected with LPS and treated with saline (LPS – SAL, n=10), runner plasma (LPS – RP, n=10), runner plasma without CLU (LPS – RP – CLU, n=8), runner plasma without FH (LPS – RP – FH, n=8), runner plasma without LIFR (LPS – RP – LIFR, n=8)) or runner plasma without PDEF (LPS – RP – PDEF, n=8). Heat map representing the relative differences between the mean of each group (zscore of means) on selected inflammatory gene markers in the hippocampus. b. Western blotting shows that CLU removal from a plasma sample after depletion via CLU antibody coupled to superparamagnetic beads using a Dynabeads® Antibody Coupling Kit bind to CLU.
