Fig. 1: SARS-CoV-2-specific T cells in SN-HCWs.
From: Pre-existing polymerase-specific T cells expand in abortive seronegative SARS-CoV-2
a, Design of the HCW and prepandemic cohorts. nAb, neutralizing antibodies. b, Cycle threshold values for the E gene PCR analysis in SN-HCWs and HCWs with a laboratory (lab)-confirmed infection (undetectable at 40 cycles was assigned 41). c, d, Anti-spike S1 (c) and anti-NP antibody (d) titres in SN-HCWs (baseline to week 16; n = 58; dotted lines at assay positivity cut-off and at average peak (AvPos) response in laboratory-confirmed infection). e, Pseudovirus neutralization at week 16. The crossed circles represent individuals who were excluded from SN-HCW group (IC50 > 50). f, SARS-CoV-2 proteome highlighting RTC and structural regions assayed for T cell responses (peptide subpools are identified by the numbered boxes) and the number of overlapping 15-mer peptides (or mapped epitope peptides (MEP) for spike). g–j, IFNγ ELISpot analyses. g, h, Viral proteins recognized by individuals coloured by specificity (g) and the number of viral proteins targeted by group (h). i, j, The magnitude of the T cell response coloured by viral protein (i) and the cumulative magnitude of the T cell response by group (j). The red bar shows the geometric mean. For e, h, the red bar shows the median. For h, j, statistical analysis was performed using Kruskal–Wallis tests with Dunn’s correction. M, membrane; SFCs, spot-forming cells. For b–e, g–j, participants were from the COVIDsortium HCW cohort.
