Extended Data Fig. 3: Glucose uptake of SGLT1 mutants.
From: Structure and mechanism of the SGLT family of glucose transporters
a, Uptake activities of SGLT1con and SGLT1conHA. SGLT1conHA is the same as SGLT1con, except that W660 and G661 of SGLT1con are reversed to H660 and A661. The uptake buffer contains different combinations of sodium (Na), choline (Ch), or phlorizin (Pz) as indicated. Uptake of αMDG is shown (mean ± SEM; n=4 biological replicates). b, Uptake activities of SGLT1 and SGLT1-WG mutant, in which H660 and A661 are substituted with W660 and G661 (mean ± SEM; n=4 biological replicates). c, Uptake activities of SGLT1 with mutations in the cholesterol-binding site (mean ± SEM; n=4 biological replicates). d, Thermostability of SGLT1con (left) or SGLT1 (right) and their variants under conditions with or without cholesteryl hemisuccinate (CHS) (mean ± SEM; n=3 biological replicates). e, The transport of αMDG by SGLT1 WT (left) and SGLT1conHA (right) in the presence of various concentrations of αMDG. Data were plotted according to the equation, U=Umax x [S]/ (K0.5+[S]) (mean ± SEM; n=3 biological replicates).
