Extended Data Fig. 9: Complementation of rice defective in strigolactone biosynthesis by grafting mutants onto wild-type rootstocks.
From: Monocotyledonous plants graft at the embryonic root–shoot interface
a, Position of the carotenoid cleavage dioxygenase 8 (OsCCD8, Os01g0746400) gene in the rice strigolactone biosynthesis pathway. Red color indicates the homozygous mutant ccd8/ccd8 allele (the d10 mutant44). b, Height of the tallest ligule (upper plot), and length of the longest leaf (lower plot) on each grafted plant measured over time. Statistically significant differences for tallest ligule height and longest leaf length were assessed by two-way repeated measures ANOVA among graft types over time. Letters indicate significant (P < 0.05) groupings among graft types at each time point using pairwise t-tests with adjusted P values by the Bonferroni multiple testing correction method. ns = not significant. Data are presented as mean ± s.d. n=8. c, Shoot phenotypes of initial grafts (upper panel) and their offspring derived from self-pollination (lower panel) after 40 days of growth in soil. d, Rates of reversion to a high tillering (mutant) phenotype in the offspring of self-pollinated grafts between ccd8 mutant (d10) and wild type (O. sativa var. Shiokari). Although the mutant phenotype was rescued when grafted to a wild type root system, offspring of the mutant scions from such grafts reverted to the mutant phenotype, as expected. Scale-bars represent 10 cm (c).
