Extended Data Fig. 1: Discovery of a method that allows grafting of monocotyledons.
From: Monocotyledonous plants graft at the embryonic root–shoot interface
Immature embryos of wheat (T. aestivum - orange) and pearl millet (P. glaucum - blue) were grown in tissue culture to regenerate a fused plant which may simulate grafting. a-d, Grafted plants were not generated after simulating grafting by placing halved calli in contact (a), pressing two scutella together in media (b), slicing scutellar tissue and placing into close contact (c), or placing callus into a toroidal arrangement (d). However, after removal and exchange of the central part of immature wheat and pearl millet embryos, some germinated into what appeared to be vestigial grafted plants (e). Photographs of tissue (left) are next to graphical representations (right). White arrowheads indicate areas of fusion. Scale-bar represents 0.5 cm and applies to all photographs.
