Extended Data Fig. 7: Overview of TF binding and perturbation experiments.

a. Table showing a summary of all TF binding/perturbation experiments indicating number of affected regions, top motif enrichment and overlap with deep learning binding sites. b. CUT&Tag signal of Repo is enriched over predicted Repo binding sites. c. CUT&Tag signal of Ey is enriched over predicted Ey binding sites. d. Optimization of CUT&Tag for Ey, finding a combination of Pitstop and higher Tn5 concentration to increase the number of regions detected and improve motif enrichment. e. The genomic region near trio contains peaks for both glia and Kenyon cells, with predicted binding sites for Ey and Repo. Ey and Repo CUT&Tag data are normalized against each other, showing biases to the Ey side over Ey binding sites and to the Repo side over Repo binding sites. f. TaDa coverage of Mef2 in optic lobe (Tm1) and Kenyon cells (left); and of Mef2 in γ-KC for predicted Mef2 regions in the optic lobe and the γ-KC. g. Venn diagrams showing overlap of TaDa experiments for Mef2 (left) and Acj6 (right). Motif enrichment is shown with Mef2 motifs enriched in all overlaps of Mef2, but no Acj6 enrichment in unique regions for acj6-TaDa. Strongest enrichments are found for common regions. h. Summary of RNAi results of Fig. 3f (knockdown in γ-KC and T4/T5 neurons). Bar plots show affected regions in both directions upon knock-down, with expected direction accentuated. Enriched motifs for the unexpected direction are shown. i–k. Results of hypergeometric tests of the overlap of TF knockdown affected regions for cistromes (i), cell-type specific cistromes (j) and deep learning binding sites (k). l. Mamo RNAi ATAC peaks from γ-KC are enriched for α/β Kenyon cell regions compared to WT or other knockdowns. m. Examples of three loci where Mamo RNAi has led to increased accessibility of α/β Kenyon cell regions in γ-KC.