Extended Data Fig. 6: Transitional states of neutrophils in vessels.
(A) Heatmap of all parameters across all behaviors, including the three sub-groups in B2. (B) UMAP based on hierarchical clustering to identify two additional behavioral clusters within B2. (C) Distribution of cells in each sub-cluster B2.1, B2.2 and B2.3 for the indicated parameters, showing for example that cells B2.3 feature sizes and distances to the vessel wall similar to those of B3. Data analyzed by one-way ANOVA. (D) Transitions between behavioral clusters shown graphically in the UMAP (left) and quantified at right. (E) Scheme illustrating the most common transitions typically involving passage through B2, suggesting that this is an obligate transitional stage for neutrophils in inflamed vessels. Drawings in each group represent the silhouettes of representative cells at different times as in Fig. 2h.
