Extended Data Fig. 9: Association of the P681R mutation with sensitivity to NAbs.
From: Enhanced fusogenicity and pathogenicity of SARS-CoV-2 Delta P681R mutation
a, Neutralization assay using three monoclonal antibodies (clones 8A5, 4A3 and CB6). Assays were performed in triplicate. b,c, Neutralization assay using 19 vaccinated sera. Pseudoviruses and effector cells (S-expressing cells) were treated with serially diluted NAbs or sera as described in Methods. Assays were performed in triplicate. The raw data of panel b are shown in panel c. NT50, 50% neutralization titre. In b, each dot indicates the mean NT50 value of the respective donor. A statistically significant difference versus the D614G virus was determined by two-sided Wilcoxon matched-pairs signed rank test. In c, the NT50 values of D614G S (black) and D614G/P681R S (orange) for each serum are indicated in each panel.
