Extended Data Fig. 5: Features of anchor-targeting MBCs and EMPEM 2D classes. Related to Fig. 3.
From: Broadly neutralizing antibodies target a haemagglutinin anchor epitope
a, 33 mAbs with anchor epitope-binding mAb repertoire features were generated and tested for competing for binding with 047-09 4F04. b, c, Number of heavy chain mutations (b) and isotype usage (c) of B cells with repertoire features of anchor-binding mAbs (n = 119 cells) or utilize VH1-69/kappa (n = 365 cells). d, e, 2D class averages of pAbs from donors 236 (d) and 241 (e) at days 7 and 14 post immunization binding to A/Michigan/45/2015 HA (×62,000 normal magnification). The last row of 2D classes in d is HA monomer complexes processed independently from trimer complexes. Data in b are represented as mean ± S.D. Data in b were analyzed using a two-tailed paired non-parametric Wilcoxon matched-pairs signed rank test.
