Extended Data Fig. 4: Expression patterns and chromatin landscapes of genes around the large deletion.
From: Genome-edited powdery mildew resistance in wheat without growth penalties
a, Expression of TaTMT3B and 11 other nearby genes in different tissues; data are from a previous publication30. Genes within the deleted region are indicated. b, c, Amino acid sequence alignment between homeologs on the A, B and D genomes of downregulated genes in the deletion region. d, Expression levels of TaTMT3B in different tissues of Tamlo-R32 and WT plants measured by quantitative RT-PCR. Results are normalized to TaPAGE gene. n.d., not detected. Data are means ± s.d., of three independent RNA preparations from biological replicates. e, Chromatin accessibility, and histone modification profiles in the TaTMT3B-MLO-B1 region in leaf tissue of Chinese Spring wheat. The Integrative Genomics Viewer (IGV) views show the various chromatin status profiles near TaTMT3B. The y-axis represents signal enrichment computed from reads at each position normalized to the total number of reads (RPKM). The dark shading indicates regions with either repressive (H3K27me3) or active (H3K4me3, H3K36me3, H3K27ac) histone modifications (a-f). f, Schematic illustration of a possible model for regulation of the activation of TaTMT3B expression in Tamlo-R32
