Extended Data Fig. 10: Examples of kyklonas in three independent cohorts.

a, A single undifferentiated sarcoma genome depicting the overlap of kataegis with ecDNA regions displayed as a rainfall (left) with a single zoomed in ecDNA represented using a circos plot (middle). The outer track of the circos plot represents the reference genome of the ecDNA with proximal known cancer driver genes. The middle track reflects a circular rainfall plot where each dot represents the IMD around a single mutation coloured based on the substitution change. The innermost track shows the average VAF for each kyklonic event. Right: Two smaller regions of the selected ecDNA including a single kyklonic event within ZNF536 region resulting in a plethora of missense and stop-gained mutations, and a single kyklonic event within a promoter flanking with the average VAFs per event (orange). b, A single lung adenocarcinoma genome depicting the overlap of kataegis with ecDNA regions (left) with a single zoomed in ecDNA containing TBC1D15 and two distinct kyklonic events represented using a circos plot (middle). Right: Two kyklonic events overlapping an upstream region and TBC1D15. c, A single oesophageal squamous cell carcinoma genome depicting the overlap of kataegis with ecDNA regions (left) with a single zoomed in ecDNA containing PRKAA2 and DAB1 and three distinct kyklonic events (middle). Right: Two kyklonic events overlapping DAB1.