Extended Data Fig. 4: Approach for identification of EcR and Hr3 targets using scRNA-Seq.
From: A global timing mechanism regulates cell-type-specific wiring programmes
a, Scheme for scRNA-Seq based transcriptomic analysis of WT and mutant lamina neurons (see Kurmangaliyev et al11. and methods). GFP vs forward scatter 2-D plot showing criteria used to enrich for lamina neurons by FACS is shown on the right. ‘Cells’ highlighted in purple were excluded despite being GFP+ due to their small size. b, tSNE plots showing WT L1-L5 clusters at 24, 36, 48, 60, 72, 84 and 96 hAPF (Adult). c, Log(expression) of previously identified lamina neuron type-specific genes in L1-L5 clusters identified at each time point over development (see Tan et al32.). d, Comparison of lamina neuron transcriptomes generated by scRNA-Seq in this study and by scRNA-Seq in Kurmangaliyev et al. Values given are Spearman correlation values. For all box-plots, solid line depicts median, while the upper and lower bounds of the box depict the third and first quantile of the data spread respectively.
