Extended Data Fig. 6: Monozygotic and dizygotic non-MS twin pairs facilitate dissection of genetic and environmental influences on immune composition.

a, Biaxial plots displaying the gating strategy for cells from monozygotic and dizygotic twin pairs of the non-MS twin cohort in order to obtain comparable cell populations as present in the reference framework of the MS twin cohort. b, Pie chart displaying the variance components for CD25 expression in memory Th cells (left panel) and regulatory T cells (Treg; right panel) using the structural equation model and the median CD25 expression in the non-MS twin cohort. c, Heatmap (top left panel) displays expression profile of canonical subsets detected in the validation cohort. Network visualization of unsupervised FlowSOM nodes of the validation cohort and mapping onto the manually-annotated reference framework (grey dots; bottom left panel). Annotation provided in the publicly available mass cytometry dataset was used to match the main clusters in the reference nodes and indicated by the respective color. Dot size corresponds to population frequency among total leukocytes. Violin plots (right panel) showing the frequency of major canonical immune subsets in the validation cohort for HD (n = 29) and RRMS patients (n = 30). d, Violin plots showing the frequency of annotated Th cell nodes in the validation cohort for HD and RRMS patients (right panel). Heatmap (left panel) displaying the expression profile for the indicated Th cell populations. Violin plots contain a bold horizontal line depicting the respective group mean. If not indicated, differences between experimental groups were statistically not significant (p > 5%) using a two-sided unpaired nonparametric Mann-Whitney-Wilcoxon test with a false discovery correction according to the Benjamini-Hochberg approach.