Extended Data Fig. 7: Effect of innate immune sensors on endothelial cell response after SARS-CoV-2 infection in the LoC model.
From: The cGAS–STING pathway drives type I IFN immunopathology in COVID-19
a, Representative images of the epithelial and endothelial cells on LoC infected with SARS-CoV-2 at 3 dpi (above). Western blot characterization of STING expression in epithelial and endothelial cells treated with control (ctrl) and STING shRNAs (below, left). Modal value of CD31 expression in the endothelial layer of LoCs reconstituted with control or STING shRNA treated epithelial or endothelial cells (below, right). Each data point was calculated from a maximum intensity projection of CD31 expression from n = 7 (sh Ctrl - epithelial/sh STING - endothelial), n = 8 (sh Ctrl – epithelial/sh Ctrl – endothelial and sh STING – epithelial/sh Ctrl – endothelial) and n = 9 (sh STING – epithelial/sh STING = endothelial) fields of view from n = 1 LoC in each case. b, Representative 3D views at 3 dpi of the vascular surface of a LoCs reconstituted with endothelial cells treated with ctrl or MAVS shRNA and infected with SARS-CoV-2. Volumes with high levels of IFNβ (bright pink) are shown as surfaces. Quantification of the volume with high IFNβ expression from ctrl (n = 5 fields of view) and MAVS shRNA (n = 4 fields of view) from n = 1 LoC in each case. Reduction of MAVS mRNA in endothelial cells after shRNA transduction (right). ‘2C’ refers to 2-cell component (epithelial cells, endothelial cells) LoCs. Bars represent mean ± s.e.m.; P values were calculated by a one-way ANOVA test followed by Tukey multiple comparison tests (a) or a two-tailed Mann–Whitney test (b). For gel source data, see Supplementary Fig. 1.
