Extended Data Fig. 7: Ratiometric calcium imaging of ellipsoid body ring neuron activity in CS-only and US-only conditions.
From: Differential mechanisms underlie trace and delay conditioning in Drosophila
a, Ratiometric imaging of EB1-Gal4, UAS-GCaMP6f.myr-tdTomato female during a single CS-only presentation (no US) experiment. Shown, raw dFratio/Fratio activity traces (where Fratio = FGCaMP6f/FtdTomato) for trials 1, 4, and 7. Red curve fits are single-term exponential model fits through the raw dFratio/Fratio activity traces starting at the end of CS. b, Exponential curve fit decay rates of EB1 neural activity post-CS. c, Normalized relative power of EB1 neural activity for trials 1, 4 and 7 (left to right) during CS. Shown are the detected frequencies at maximum relative power greater than peak mean baseline frequency. Black line represents mean baseline frequency spectrum (from n = 51 flies). d, Frequency (at maximum relative power) of EB1 neural activity during CS (see Methods, ‘Frequency and decay rate computation’). e, Normalized relative power of EB1 neural activity for trials 1, 4 and 7 (left to right) during the 5 s post-CS period. Shown are the detected frequencies at maximum relative power greater than peak mean baseline frequency. Black line represents mean baseline frequency spectrum (from n = 51 flies). f, Frequency (at maximum relative power) of EB1 neural activity during the 5 s post-CS period (see Methods, ‘Frequency and decay rate computation’). CS-only presentation trials, n = 17 flies. Box plot centre (median), edges (IQR), whiskers (1.5x IQR). Scatters represent individual fly activity scores. Friedman’s repeated measure ANOVA test was used for comparisons between trials. No significant difference was observed. g, Ratiometric imaging of an EB1-Gal4, UAS-GCaMP6f.myr-tdTomato female during a single US-only presentation (no CS) experiment. Shown, raw dFratio/Fratio activity traces (where Fratio = FGCaMP6f/FtdTomato) for trials 1, 4, and 7. No notable calcium activity was detected. h, Normalized relative power of EB1 neural activity for trials 1, 4 and 7 (left to right) during the 10 s CS fictitious presentation (5–15 s). No frequencies of maximum relative power greater than peak mean baseline frequency were detected. Black line represents mean baseline frequency spectrum (from n = 51 flies). i, Normalized relative power of EB1 neural activity for trials 1, 4 and 7 (left to right) during the 5 s post-fictitious-CS presentation (15–20 s). No frequencies of maximum relative power greater than peak mean baseline frequency were detected. Black line represents mean baseline frequency spectrum (from n = 51 flies). US-only presentation trials, n = 8 flies.
