Extended Data Fig. 9: Ratiometric calcium imaging of ellipsoid-body-projecting dopaminergic neurons in CS-only and US-only conditions.
From: Differential mechanisms underlie trace and delay conditioning in Drosophila
a, Ratiometric imaging of a c346-Gal4, UAS-GCaMP6m.myr-tdTomato female during a single CS-only presentation (no US) experiment without (air puff) distractors (left), and with distractors (right). Shown, raw dFratio/Fratio activity traces (where Fratio = FGCaMP6m/FtdTomato) for each training trial. b, Peak dFratio/Fratio activity of ellipsoid body DANs during CS (top), and post-CS (bottom), across trials, without distractors (blue, n = 5 flies), and with distractors (red, n = 5 flies). c, Ratiometric imaging of a c346-Gal4, UAS-GCaMP6m.myr-tdTomato female during a single US-only (no CS) experiment without (air puff) distractors (left), distractors with US in a simulated delay conditioning setting (middle), and distractors with US in a simulated trace conditioning (5-s TI) setting (right). Shown, raw dFratio/Fratio activity traces (where Fratio = FGCaMP6m/FtdTomato) for each training trial. d, Peak dFratio/Fratio activity of ellipsoid body DANs prior to US (left), and post-US (right), across trials, without distractors (black, n = 5 flies), distractors with US in simulated delay conditioning setting (blue, n = 5 flies), and distractors with US in simulated trace conditioning (5-s TI) setting (red, n = 5 flies). Box plot centre (median), edges (IQR), whiskers (1.5x IQR). Scatters represent individual fly activity scores. Group comparisons were performed using a two-factor ART-ANOVA test. No significant difference was observed between groups.
