Extended Data Fig. 10: Role of Dop1R2 and DopEcR dopamine receptor signalling in ellipsoid body ring neurons during delay and trace conditioning.
From: Differential mechanisms underlie trace and delay conditioning in Drosophila
a, Frequency (with maximum power, see Methods, ‘Frequency and decay rate computation’) of EB1 neural activity with either Dop1R2 or DopEcR dopamine receptor signalling impairment across trials for delay conditioning during CS for EB1-Gal4, UAS-GCaMP6f.myr-tdTomato flies (black), EB1-Gal4, UAS-GCaMP6f.myr-tdTomato, UAS-Dop1R2RNAi flies (red), and EB1-Gal4, UAS-GCaMP6f.myr-tdTomato, UAS-DopEcRRNAi flies (blue). n = 9 flies (black), n = 5 flies (red), n = 5 flies (blue). b, Frequency of EB1 neural activity with either Dop1R2 or DopEcR signalling impairment across trials for trace conditioning (5-s TI) (during CS, left; during TI, right) for EB1-Gal4, UAS-GCaMP6f.myr-tdTomato flies (black), EB1-Gal4, UAS-GCaMP6f.myr-tdTomato, UAS-Dop1R2RNAi flies (red), and EB1-Gal4, UAS-GCaMP6f.myr-tdTomato, UAS-DopEcRRNAi flies (blue) (see Methods, ‘Frequency and decay rate computation’). n = 16 flies (black), n = 5 flies (red), n = 5 flies (blue). Box plot centre (median), edges (IQR), whiskers (1.5x IQR). Scatters represent individual fly metrics. Group comparisons were performed using a two-factor ART-ANOVA test. No significant difference was observed between groups.
