Extended Data Fig. 5: Time lapse of Her2 Tri-specific antibody induced T-cell mediated killing of target HER2+ MDA-MB-453 breast cancer cells. | Nature

Extended Data Fig. 5: Time lapse of Her2 Tri-specific antibody induced T-cell mediated killing of target HER2+ MDA-MB-453 breast cancer cells.

From: A trispecific antibody targeting HER2 and T cells inhibits breast cancer growth via CD4 cells

Extended Data Fig. 5: Time lapse of Her2 Tri-specific antibody induced T-cell mediated killing of target HER2+ MDA-MB-453 breast cancer cells.

HER2+ MDA-MB-453 cells were labeled with CellVue Maroon dye (Invitrogen) and combined with PBMCs in 12-well plates. HER2 trispecific Ab was added at 1 µg/ml concentration. Time lapse imaging was conducted for 24 h using a confocal microscope equipped with an environmental chamber which maintains cultures at 37 °C, 5% CO2.

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