Extended Data Fig. 4: Targeted Knockdown of Fshr in the Hippocampus Diminishes AD Pathologies.
From: FSH blockade improves cognition in mice with Alzheimer’s disease
a, Quantitative PCR shows significantly reduced expression of Fshr, Cebpb, Lgmn, App and Mapt. b, Immunohistochemistry of the hippocampus shows reduced accumulation of Aβ and pTau, as well as of proteinaceous deposits (silver staining) in si-Fshr-injected OVX mice (scale bar, 50 μm). c, The two isoforms of Aβ, namely Aβ40 and Aβ42, were also reduced. d, Notable is the marked increase in dendritic spines (Golgi staining) (scale bar, 5 μm). Statistics: mean ± s.e.m., (a) 3 biological replicates; (c) 5 mice per group; (d) 10 sections from 3 mice per group; unpaired two-tailed Student’s t-test.
