Extended Data Fig. 2: Intestinal colonization by C. albicans does not cause spontaneous colitis during homeostasis nor does it aggravate DSS-induced colitis.
From: Immune regulation by fungal strain diversity in inflammatory bowel disease
a, Faecal C. albicans burdens were assessed after 3 days of C. albicans colonization in mice that received either control feeding water (n = 4) or DSS water (n = 5) for 4 days. Dots represent individual mice. b–g, Mice, after being gavaged with PBS (n = 5) or C. albicans (n = 5) for 14 days, were induced by 3% DSS water for 7 days. b, Schematic figure of DSS-induced colitis model of mice with intestinal colonization by C. albicans. c, H&E staining and histology score of colon sections. n= 9 (DSS + control PBS) and n = 7 (DSS + C. albicans). The scale bar 200 μm. Data in c are pooled from two independent experiments with similar results. d, e, Representative flow cytometry plot and quantification of Foxp3+ (d) and RORγt+ CD4+ T (e) cells. f, g, Mice were gavaged twice per week with PBS (n = 5) or C. albicans WT SC5314 (n = 5). Mice were sacrificed four months later for colon length (f) and histology evaluation, the scale bar 100 μm (g). Each dot represents an individual mouse. Data in a–e are representative of three independent experiments with similar results. Data in f, g representative of two independent experiments with similar results. Data in a, c, and d–g are shown as mean ± s.e.m., unpaired, two-tailed, Mann-Whitney test.
