Extended Data Fig. 13: Central targets of VSNs innervating various visceral organs.
From: A multidimensional coding architecture of the vagal interoceptive system
a, Representative brainstem images at indicated Bregma levels containing central projections of VSNs retrogradely labelled from indicated visceral organs using AAVrg-tdTomato. Scale bar: 100 μm. b, Distribution of central VSNs terminals retrogradely labelled from indicated visceral organs (colour-coded) along the rostral-caudal axis. c, Brainstem area innervated by VSNs from indicated organs along the rostral-caudal axis (mean ± SEM, number of mice: 2 (oesophagus), 3 (others)). d, Percentage innervation, calculated as vagal afferent fluorescence in indicated subnuclei normalized to the fluorescence in all vagal afferent targets, for various brainstem subnuclei along the rostral-caudal axis (mean ± SEM, n as in c). e, Quantitative analysis of innervation density in indicated brainstem regions from Bregma −7.2 mm to −8.0 mm by VSNs from various visceral organs, expressed as fluorescence in indicated area/fluorescence in total area (mean, colour-coded, n as in c). f, Correlation variance matrix among organ pairs showing that VSNs from functionally related organs terminated in similar brainstem regions.
