Extended Data Fig. 10: Cerebrovascular profiling in Huntington’s disease. | Nature

Extended Data Fig. 10: Cerebrovascular profiling in Huntington’s disease.

From: Single-cell dissection of the human brain vasculature

Extended Data Fig. 10

a. UMAP of integrated single nuclei from post mortem control and HD human patient samples. b. UMAP of integrated cerebrovasculature cells in post mortem control and HD human patients. c. Comparison of cerebrovasculature cell annotations (in cell numbers) in this study vs. Lee et al. d. UMAP analysis of astrocyte subclusters in HD. Vascular-related astrocytes outlined in blue. e. UMAP analysis of microglia subclusters in HD. Vascular-related microglia outlined in blue. f. ChEA prediction of top 10 regulators of upregulated genes in HD endothelial, mural, and fibroblasts cells. g. Pathway analysis of the top 10 enriched upregulated pathways in HD endothelial, mural, and fibroblasts cells. h. PKR immunoreactivity in the R6/2 HD mouse model engulfs blood vessels with low CLDN5 expression. i. Western blots for tight junction proteins CLDN5 and TJP1 from human HD and control samples. Representative images in h. from n = 3 independent biological replicates for each immunostaining. Brightness and contrast in immunofluorescence enhanced for visualization. Scale bar, 20 μm.

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