Extended Data Fig. 1: Quantification of UvrABD binding to RNAP in vivo.

Supplementary to Fig. 1. a, UV sensitivity of E. coli strains used in Fig. 1. Representative efficiencies of colony formation of parent wild-type (MG1655) and mutant E. coli cells exposed to the indicated UV doses. Overnight cultures were diluted 1:100 with fresh LB and grown to ~ 2x10⁷. Cells were serially diluted, plated on LB agar, irradiated with UV, and incubated at 37 °C for 24 h. b, Representative western blots used to generate the plots of Fig. 1b–d. c, Quantitative mass-spectrometry analysis of RNAP-associated UvrABD, Mfd, and Rho in the exponentially grown cells prior to genotoxic stress. RNAP pulldown samples were prepared as in Fig. 1. Values are normalized to that of NusA-containing RNAPs, thus reflecting the RNAP molecules engaged in elongation in vivo.