Extended Data Fig. 3: Candidate tTF expression in neuroblast trajectory.

Expression pattern over pseudotime of all TFs that were found to be expressed in a temporal manner along the medulla neuroblast trajectory. (a) 14 transcription factors were found to be expressed temporally in high relative expression levels. These include the already known tTFs (hth, ey, slp1, slp2, D, and tll in purple), as well as eight new candidate tTFs (in green). (b) Another 25 transcription factors were found to be expressed temporally in lower relative expression levels. Ap, ct, gcm, and gem expression were tested in developing optic lobes and they were not expressed temporally. Hence, these 25 transcription factors were excluded from downstream analysis. (c) Gcm-Gal4 (green) is expressed in glial cells coming from the Tll temporal window (magenta)⁴⁶. Scale bar: 10 µm. (d) Cut-Gal4 (green) is expressed in neuroblasts of all ages found in the surface of the optic lobe (arrow). Scale bar: 10 µm.