Extended Data Fig. 3: Cryo-EM data processing procedure for the Omicron S-ACE2 complex.
From: Molecular basis of receptor binding and antibody neutralization of Omicron
a, Data processing procedure for the Omicron S-ACE2 dataset. A representative original micrograph and the reference-free 2D class averages are also presented. The masks for the focused 3D classification on RBD-2 and RBD-3 regions are shown as pink and orange transparent surfaces, respectively. b, Population distribution of the Omicron S-ACE2-C1/-C2/-C3 states. c, Lower threshold rendering of the Omicron S-ACE2-C1/-C2/-C3 maps. ACE2 density (violet red) also associates with RBD-2 or RBD-3 in the C2 and C3 states, although appearing weaker than that of the stably associated ACE2 on RBD-1, indicating a dynamic ACE2 association with RBD-2/−3.
