Extended Data Fig. 6: The involvement of BARX2 in lacrimal-gland-like organoid formation.
From: Generation of 3D lacrimal gland organoids from human pluripotent stem cells
a, Immunostaining for BARX2 (green) and KRT14 (red) in domed (upper panels) and flat (lower panels) colonies derived from sorted P3 cells. n = 3 biological replicates. Nuclei, blue. Scale bars, 100 μm. b, Colony-forming efficiency and the proportion (at day 21) of domed colonies of P3 cells sorted from SEAMs after 8 and 14 weeks of differentiation culture. n = 3 biological replicates. Data are presented as mean ± s.d. Exact p values are provided in the Source Data. c–d, Immunostaining for PAX6 (green) and KRT12 (red) in 8-week (c) and 14-week (d) differentiated SEAMs. n = 3 biological replicates. Nuclei, blue. Scale bars, 100 μm. e, Immunostaining for BARX2 (green) and PAX6 (red) in lacrimal-gland-like progenitors (left panels) and organoids (right panels). n = 3 biological replicates. Nuclei, blue. Scale bars, 100 μm. f, Effect of BARX2 knockdown using siRNA on domed-colony formation. *p < 0.01 (n = 8 biological replicates; NS, not significant). Data are presented as mean ± s.d. Exact p values are provided in the Source Data. g, Effect of BARX2 knockdown using siRNA on corneal epithelial differentiation. n = 1 sample for each experimental condition. Nuclei, blue, Scale bars, 100 μm.
