Extended Data Fig. 7: Differentiation capability of SEAM-derived lacrimal-gland-like organoids and corneal epithelial cell sheets.
From: Generation of 3D lacrimal gland organoids from human pluripotent stem cells
a, 3D organoid culture using cells from SEAM-derived corneal epithelial cells. n = 8 biological replicates. Scale bars, 1,000 μm. b, 2D corneal epithelial culture using cells from lacrimal-gland-like organoids. n = 19 biological replicates. Scale bars, 1,000 μm. c–f, Immunostaining of cells derived from lacrimal-gland-like organoids after 2D cultivation as shown in (b): (c) CD44 (green) and KRT12 (orange); (d) PAX6 (green) and p63 (orange); (e) AQP5 (green), KRT13 (orange), and KRT14 (magenta); (f) HTN1 (green), SOX9 (orange), and KRT3 (magenta). n = 3 biological replicates in (c) - (f). Nuclei, blue. Scale bars, 100 μm. g, Prolonged 3D cultivation of lacrimal-gland-like organoids up to 41 days. n = 2 biological replicates. Scale bars, 1,000 μm. h, Cell passaging of lacrimal-gland-like organoids. n = 2 technical replicates. Scale bars, 1,000 μm.
