Extended Data Fig. 10: Comparison of exocrine gland cells.
From: Generation of 3D lacrimal gland organoids from human pluripotent stem cells
a, Immunostaining for CD44 (green), AQP5 (red) and PAX6 (magenta) in lacrimal gland (rat), salivary gland, mammary gland and pancreas (all humans). Lower panels show magnified views of the upper panels. Dotted lines in pancreas tissue indicate the presumptive region of islets. n = 3 biological replicates (rat lacrimal gland) and n = 1 (human gland tissue). Nuclei, blue. Scale bars, 100 μm. b, Expression of PAX6, AQP5 and CD44 in human exocrine gland tissues, lacrimal glands (n = 8), salivary glands (n = 5), and mammary glands (n = 8) based on existing microarray datasets. c, Expression of lacrimal gland and ocular surface epithelium-related genes in transplanted lacrimal-gland-like organoids (tLGO) and in human corneal and conjunctival epithelial cells. CE, corneal epithelial cell; CjE, conjunctival epithelial cell. tLGO, n = 4; CE, n = 6; CjE, n = 3 biological replicates. Data are presented as mean ± s.d. d, Summary of expression of lacrimal gland-related markers in glandular tissues and ocular surface epithelium. Data indicate that PAX6, AQP5, LTF and LYZ-expressing cells are lacrimal gland type cells. SG, salivary gland; MG, mammary gland; PC, pancreas; ND, not determined.
