Extended Data Fig. 3: Characterization of lacrimal-gland-like organoids.
From: Generation of 3D lacrimal gland organoids from human pluripotent stem cells
a–c, Light sheet fluorescence microscopy of lacrimal-gland-like organoids stained with CDH1 and PAX6 (a), KRT14 (b) and CD44 (c). Lower panels show magnified views of the upper panels. n = 2 biological replicates. Scale bars, 200 μm. d, H&E staining of hiPSC-derived lacrimal-gland-like organoids. n = 3 biological replicates. Scale bars, 500 μm. e, Immunolocalization of PAX6 (green) and CDH1 (red) in thin sections of hiPSC-derived lacrimal-gland-like organoids. n = 3 biological replicates. Nuclei, blue. Scale bars, 100 μm. f, Immunostaining for SOX9 (green), AQP5 (red) and ACTA2 (magenta) in thin sections of hiPSC-derived lacrimal-gland-like organoids. n = 3 biological replicates. Nuclei, blue. Scale bars, 100 μm. g–i, Immunostaining of KhES-1-derived lacrimal-gland-like organoids for PAX6 (green) and CDH1 (red) (g), CD44 (green), HTN1 (red) and CDH1 (magenta) (h), and SOX9 (green), AQP5 (red) and ACTA2 (magenta) (i). n = 3 biological replicates. Nuclei, blue. Scale bars, 100 μm. j, Gene expression analysis for lacrimal gland-related markers in iPS cells (iPSC), P3 cells, lacrimal-gland-like progenitors (LGP) and organoids (LGO). n = 4 biological replicates. Data are presented as mean ± s.d.
