Extended Data Fig. 4: Characterization of iPS cell-derived lacrimal-gland-like organoids by single-cell RNA sequencing.
From: Generation of 3D lacrimal gland organoids from human pluripotent stem cells
a, Uniform manifold approximation and projection (UMAP) plot of all single cells and the summary of enriched GO terms in each cluster; n = 4071 cells (day 0), n = 4566 cells (day 10), n = 2448 cells (day 20). b, Pseudotime trajectory of the single-cell transcriptome showing three branches from the root (cluster 0 of day 0). These represent active epithelial-mesenchymal transition (EMT), cell proliferation, and the development of epithelial secretory cells. c, Cell populations preferentially expressed lacrimal gland-related genes. d, Cell-type annotations of lacrimal-gland-like organoids (day 20) with positive and negative marker genes.
