Extended Data Fig. 7: Immunofluorescent detection of nucleotide oxidation in neurons.
From: Somatic genomic changes in single Alzheimer’s disease neurons
Immunofluorescence was performed on post-mortem human brain prefrontal cortex. NeuN (AF488) was used to label neurons and 8-oxoG (AF555) used to label oxidized guanine nucleotides. a, For each case sample, in a full microscopic field of up to 100 NeuN+ neurons, 8-oxoG signal was quantified per neuron. Here, each data point represents the 8-oxoG signal from one neuron, with mean and SEM shown in black for each case. Figure 2f shows mean 8-oxoG values of each case in relation to age and disease status. b, Representative microscopy images (turquoise or purple boxes) are shown for neurotypical control and AD samples from a. n = 100 total neurons examined (50 neurons each from two independent staining experiment batches per case). NeuN+ neurons are shown in green and 8-oxoG in greyscale or magenta. Scale bars represent 60 µm.
