Extended Data Fig. 8: The impact of BHB on intestinal epithelial DNA methylation.
a, Hypothetical model by which HOPX is induced through BHB-mediated methylation changes. b–d, PCA plots (b, c) and scatter plot (d) of bisulphite sequencing of intestinal epithelial cells from mice treated with BHB or fed a KD compared to controls. e, Heatmap of CpG methylation status in the Hopx locus determined by bisulphite sequencing of intestinal epithelial cells from mice treated with BHB or fed a 90% fat diet. f, Transcript abundance of Hopx in organoids treated with BHB and/or 5-azacitidine (5-Aza). g, h, Size of wild-type (g) and Hopx-deficient organoids (h) after treatment with BHB and/or 5-Aza. i, j, Images of wild-type (i) and Hopx-deficient (j) organoids treated with BHB and 5-Aza. Error bars indicate means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Exact n and p-values are presented in Supplementary Table 3.
