Extended Data Fig. 9: A CRISPR-based approach to identify mediators of Hopx induction by BHB.
a, Hypothetical model by which HOPX is induced through BHB-mediated G protein-coupled receptor signalling. b–g, Expression of the indicated genes in organoids after CRISPR-mediated targeting thereof. h–m, Representative images (h) and size quantitation (i-m) of organoids treated with BHB after CRISPR-mediated targeting of the indicated genes. n, Schematic of organoid growth from Hcar2-deficient mice, cultured with BHB, butyrate, or vorinostat. o, Representative images of organoids from wild-type and Hcar2-deficient mice treated with BHB. p–v, Transcript levels of Hopx in organoids treated with BHB after CRISPR-mediated targeting of the indicated genes. Error bars indicate means ± SEM. **** p < 0.0001. Exact n and p-values are presented in Supplementary Table 3.
