Extended Data Fig. 7: Immunoblot analysis of TMEM106B expressed in E. coli establishes the specificity of antibody TMEM239.
From: Age-dependent formation of TMEM106B amyloid filaments in human brains
a. Diagram of the TMEM106B sequence, coloured in accordance with Fig. 1, with the immunogen of antibody TMEM239 (residues 239–250) indicated. b. Coomassie blue-stained gel and immunoblot (antibody TMEM239) of recombinant C-terminal TMEM106B fragment (WT; residues 120–274) and the immunogen-deletion construct (Δ239-250). Pellets from 1 ml cell extracts and TMEM106B purified from inclusion bodies (see Methods) were used. Red arrows indicate TMEM106B bands; red asterisks indicate non-specific binding.
