Extended Data Fig. 7: Distinct pattern of CNS nuclei participates in the formation of the ABC.

a, Schematic of PRV injection site in the perirenal abdominal aorta. b, Schematic of polysynaptic PRV retrograde migration from ATLO to brain. c, In-situ detection of India ink around the abdominal aorta and in RLN (left), but not in the kidney (right). Histological detection of ink in the adventitia, paraaortic adipose tissue (arrow) and within RLN (arrowhead), but not in the PvaG soma 2 days post-injection (n = 5 aged WT). d, PRV⁺ neurons (arrow) in TH⁺ sympathetic PvaGs at d2 or SycGs at d4 p.i., and their quantification. e, PRV⁺ neurons in thoracic 6–13^th DRGs of aged Apoe^−/− mice at d4; n = 4 per time point. f, Mapping of PRV⁺ neurons from ATLO to brain until d4. n = 4 for PvaG, 3 for CG; 5 for IML; 6 for RPa; 6 for PVN. Each circle represents mean of PRV⁺ neurons, arrow indicates direction of PRV migration, and colour indicates the p.i. day. g, Quantification PRV⁺ neurons in 27 distinct brain nuclei at 4–6d after abdominal aorta inoculation; n = 6 per time point. h, Anatomical locations of brain nuclei in g according to the Allen Mouse Brain Atlas (sagittal view). i, Connectivity mapping of PRV migration over time in 28 different neural tissues (from peripheral ganglia to higher brain nuclei depicted in f,g) after abdominal aorta inoculation. j, Anatomical representation (sagittal view) of neural tissues in i according to the Allen Mouse Brain Connectivity Atlas. Each circle represents mean of PRV⁺ neurons, arrow indicates direction of PRV migration, and colour indicates the p.i. day. k,l, Quantitative comparisons of PRV⁺ neurons in IML of the spinal cord and 27 distinct brain nuclei (k), and their anatomical mapping (l) at 6d p.i. into the abdominal aorta versus control target tissues: right eye, psoas major lumbar muscle, kidney, and blood; n = 4 per group. Scale bars, 50 µm. Data are means ± s.e.m. n represents biologically independent animals. Mixed-model ANOVA with Bonferroni post hoc test (d,e). Abbreviations: gigantocellular reticular nucleus-alpha (GiA), raphe pallidus (RPa), lateral paragigangtocellular nuclei (LPGi), lateral reticular nucleus (LRt); Raphe obscurus nucleus (Rob); rostroventral lateral medulla (RVLM); area postrema (AP); repositus nucleus (Pr); locus coeruleus (LC); Barrington’s nucleus (Bar); noradrenaline cell group 5 (A5); laterodorsal tegmental nucleus (LDTg); ventrolateral periaqueductal gray (VLPAG); lateral periaqueductal gray (LPAG); medial lemniscus (ml); peduncular part of lateral hypothalamus (PLH); dorsomedial hypothalamic nucleus (DM); ventromedial hypothalamic nucleus (VMH); arcuate hypothalamic nucleus (Arc); suprachiasmatic nucleus (Sch); cortical amygdala (CoA); lateral amygdala (LA); medial amygdala (MeA); central amygdala (CeA); intergeniculate leaflet of the thalamus (IGL); and piriform cortex (Pir)

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