Extended Data Fig. 1: Long range promoter-promoter connectivity is a pervasive feature of the Drosophila genome.
From: Transcriptional coupling of distant regulatory genes in living embryos
a, Promoter-promoter interaction distances distribution of connected genes. b–g, Micro-C contact map of the inv/en (b), slp1/slp2 (c), odd/sob/drm48 (d), pyr/ths11 (e), E5/ems (f) and nub/pdm2 (g) loci. Below, aligned to the map, are auto-scaled ChIP-seq tracks for Zelda (3h embryo)63 in red, Cohesin RAD21 (Kc167 cells)64 in blue, CP190 (Kc167 cells60), CTCF (Kc167 cells)60 in green, and in orange: Pc (2-4h embryos)65, Pho (3rd instar larva)66, Ph (3rd instar larva66), Cg (3rd instar larva)67, CLAMP (Kc167 cells)68 and GAF (2–4 h embryo21). The orange tracks correspond to proteins that show binding at the anchors of promoter-proximal regions displaying high connectivity (tethering elements). A schematic representation (to scale) of the locus is displayed below, with in situ images showing the overlapping expression pattern between the paralogue genes15,16,69,70 and a reporter line of the putative shared enhancers49.
