Extended Data Fig. 1: Identification of SCREWs in Arabidopsis.
From: Phytocytokine signalling reopens stomata in plant immunity and water loss
a, Upregulation of Arabidopsis peptide genes by flg22 in an FLS2-dependent manner. The gene expression data were extracted from an RNA-Seq analysis17 and subjected to data adjustment by log2 transformation using TBtools for the heat map. b, Phylogenetic analysis of Arabidopsis SCREW homologs and schematic diagrams of different domains. The phylogenetic tree was constructed with MEGAX using neighbour-joining methods. The bootstrap values from 1,000 replications are indicated on the branches. c, SCREW orthologs are present in dicots and monocots. Protein sequences were blast-searched against the NCBI database, and wheat, rice, and maize genomes using Arabidopsis SCREW1 as a query. The phylogenetic tree was constructed as indicated in (b) and displayed with iTOL v5 online software (https://itol.embl.de/). The bootstrap values from 1,000 replications are shown on the branches. d, SCREWs are upregulated after elf18 treatments. Ten-day-old plate-grown seedlings were treated with 200 nM elf18. The expression of SCREWs normalized to UBQ10 was analysed by RT–qPCR. Means (n = 4, biologically independent samples) of fold induction shown as log2 values were used to construct heat map using TBtools. e, SCREWs are upregulated after Pep1 treatments. Ten-day-old plate-grown seedlings were treated with 200 nM Pep1. The expression of SCREWs was detected and shown as in (d) (n = 4, biologically independent samples). f, SCREWs are upregulated after Pst DC3000 infections. Ten-day-old plate-grown seedlings were treated with Pst DC3000 at OD600 nm = 0.01, and gene expression was analysed as in (d) (n = 3, biologically independent samples).
