Extended Data Fig. 6: Crystallographic and mass spectrometry characterization of the TGA3 NID–palmitate complex. | Nature

Extended Data Fig. 6: Crystallographic and mass spectrometry characterization of the TGA3 NID–palmitate complex.

From: Structural basis of NPR1 in activating plant immunity

Extended Data Fig. 6: Crystallographic and mass spectrometry characterization of the TGA3 NID–palmitate complex.

a, Crystal structure of the TGA3 NID dimer. The two TGA3-NID molecules are shown in the rainbow colour, with N-terminus coloured in blue and C-terminus coloured in red. b, A zoomed-in view of the location of the palmitate. Polar interactions with the carboxylate group of the palmitates are indicated with dashed lines. For clarity, only one TGA3 molecule is coloured in rainbow, and the other molecule is coloured in grey. Purple meshes in panels a, b represent 2mFo-DFc omit map of the palmitate plotted at the 1.0 σ level. c, Mass spectrometry analysis of the fatty acid extracted from the protein sample, verifying the fatty acid as the palmitic acid (C16:0).

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