Extended Data Fig. 3: NPR1 harbours a unique zinc finger.
a, X-ray fluorescence scanning data revealed the presence of Zn2+ in NPR1(ΔSBD) crystals. Scanning results for the NPR1 protein crystal and buffers are shown in the left and right panels, respectively. b, Sequence alignment of BTB domains. Conserved cysteine and histidine residues in a unique cysteine cluster preserved in NPR proteins are highlighted in pink. Dots indicate residues participating in zinc coordination, and triangles denote residues mutated in npr1(dim). Listed plant species include: Arabidopsis thaliana (At), Brassica rapa (Br), Brassica juncea (Bj), Brassica napus (Bn),Raphanus sativus (Rs), Oryza sativa (Os), Nicotiana tabacum (Nt), Populus trichocarpa (Pt), Zea mays (Zm), Solanum lycopersicum (Sl), Vitis vinifera (Vv), Hordeum vulgare (Hv), Medicago truncatula (Mt), and Glycine max (Gm).
