Extended Data Fig. 2: Phototransduction sensitivity and kinetics in relation to enucleation delay.

Example plot showing how dim flash response sensitivity is calculated (a). Responses normalized to maximal response of retina plotted against the intensity of light stimulus, curve fits Naka-Rushton function (Eq. 2) to amplitude data, dim flash sensitivity is defined as response amplitude divided by R_max at constant intensity producing ~20% of the maximal response amplitude. Dim flash sensitivities of retinas from wild-type mouse retinas with different enucleation delays (b), and I_1/2 values from retinas of wild-type mice with different enucleation delays (c). Example response plot showing how I_1/2 was obtained (d), responses of retina to each intensity presented is plotted and a Naka-Rushton fit applied to the plot. I_1/2 is the intensity required to produce half the maximal response size. Amplification constant plotted against enucleation delay for each retina (e). Time-to-peak (t_p) and integral (T_i, inset) of dim responses for each retina plotted against the enucleation delay (f, *** indicates p < 0.001 compared to 0min). Exact p values: t_p: 0 vs 90 min p = 0.0002, 0 vs 120 min p = 0.002, 0 vs 180 min p = 0.001, T_i: 0 vs 90 min p = 0.002, 0 vs 120 min p = 0.0007, 0 vs 180 min p = 0.01). Example dim flash response showing how time-to-peak (t_p) and integration time (T_i) was calculated (g). Time-to-peak is the time from light stimulus onset to the peak of the dim flash response. Integral (T_i) is the area under the dim flash response curve (orange shaded area), divided by the response amplitude. Light stimulus is indicated with a shaded yellow area. For all plots, bar height indicates mean, error bars represent ± SEM. All comparisons are one-way ANOVA with Holm-Bonferroni means comparisons. (DF: 35 for all comparisons, F values: Ti = 8.4, t_p = 5.8, Dim flash sensitivity = 2.0, Amplification constant = 1.6, I_1/2 : 2.2).