Extended Data Fig. 8: Determination of active concentration of MPG.
From: Genetic and chemotherapeutic influences on germline hypermutation
(a) Representative native gel electrophoresis with 20 nM pyrolidine-DNA (Y•T) and varying concentration of WT or A135T MPG (25 mM NaHEPES pH 7.5, 100 mM NaCl, 5% v/v glycerol, 1 mM EDTA, 1 mM DTT). Agarose gels (2% w/v) were run in 0.5X TBE buffer at 10 V/cm at 4 °C. (b) Independent dilutions were fit to a binding titration to yield an active fraction of 0.57 for both WT and A135T (n = 3). This demonstrates that equal concentrations of WT and A135T were tested in the glycosylase assays. The concentrations listed are not corrected by this factor. The points shown are the mean and error bars show 1 standard deviation.
