Extended Data Fig. 5: Gibbin regulates gene expression by maintaining local enhancer–promoter chromatin architecture.

(a) Sequencing depth, PCR duplicates, and (b) valid interaction characterization of cohesin HiChIP libraries. (c) Correlation of insulation scores between WT and GKO. (d) A and B compartment scores for chromosome 1, which are representative of the entire genome. (e) Contact probability relative to contact distance for each cell type. (f) Length of contacts called by FitHiChIP in each cell type. (g) Chromatin contact matrix comparing WT and GKO cohesin HiChIP, overlaid with domains called by insulation score. (h) Contact matrix displaying the difference between GKO and WT cohesin HiChIP signal. Blue indicates greater contact in the WT, while red suggests more contact in the GKO. All matrices display chr4:110,000,000-120,000,000. (i) Overlap of contact coordinates called by FitHiChiP in either the WT and GKO. (j) Number of Gibbin-dependent chromatin contacts that are anchored in Gibbin target gene TSS’s. (k) Number of cell-type specific contacts that have one end anchored in a gene TSS (l) APA showing contact strength difference between WT and GKO cohesin Hi-ChIP at Gibbin-independent genes.